Plexin A4 is a 195kD member of the plexin A subfamily, plexin family of proteins.
It is found on sensory, autonomic and motor neurons, plus T and dendritic cells.
Plexin A4 negatively regulates cell migration, activation and axon guidance, and does so by serving as a receptor for Sema6A and 6B, and as a co-receptor with neuropilin-1 and -2 for Sema3A.
Mature human Plexin A4 is an 1871aa type I transmembrane glycoprotein.
It contains a 1214aa extracellular domain (ECD) and a 636aa cytoplasmic region.
The ECD contains one Sema-domain (aa51-482), three PSI domains (aa509-856) and four IPT regions (aa858-1230) that contain a phosphoserine at #946.
There are three isoform variants.
One shows a 65aa substitution for (aa458-1894), a second shows an 80aa substitution for (aa1292-1894), and a third shows the just mentioned 80aa substitution coupled to a 14aa substitution for (aa1-535).
Over (aa24-1237), human Plexin A4 is 97% aa identical to mouse Plexin A4.
Applications:Suitable for use in Direct ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml using SY5Y human neuroblastoma cell lineOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a lyophilized powder from PBS, pH7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human Plexin A4. Less than 5% cross‑reactivity with recombinant mouse (rm) Plexin A1, rmPlexin A2, and rmPlexin A3.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Thr24-Pro1237 from human Plexin A4, expressed in CHO cells.
