84-1190-23　PHD1 (Prolyl Hydroxylase Domain-containing Protein 1, PHD-1, DKFZp434E026, EGL Nine (C.elegans) Homolog 2, Egl Nine Homolog 2, EGLN2, Estrogen-induced Tag 6, EIT6, EIT-6, Hypoxia-inducible Factor Prolyl Hydroxylase 1, HIF Prolyl Hydroxylase 1, HIF-PH1, HIF-P4H 1, HIFPH1, HPH-3, P4H1) 100ug　P3373-02

特徴

• EGLN2 (EGL/EGg LayingNine #2; also known as PHD1 and HPH3) is a 43kD member of the EglN family of proteins.
• It is ubiquitously expressed and found principally in the nucleus.
• EGLN2 hydroxylates proline on HIF1 a.
• HIF1 is an a/b heterodimeric transcriptional activa that upregulates genes involved in mitigating the effects of hypoxia.
• Normally, and in the presence of abundant oxygen, the HIF 1 a chain is hydroxylated by PHD family members, which results in itsubiquitylation and degradation.
• Under low oxygen tension, EGLN2 activity is decreased, the HIF1 a subunit is retained, and HIF1 activates genes.
• Human EGLN2 is 407aa in length.
• It contains one iron 2oxoglutarate (Fe2OG) dioxygenase domain (aa278-376) plus an ironbinding (His297 His358), and a 2,oxoglutaratebinding (Arg367) site.
• There is one alternative start site at Met34 that generates a 40kD isoform.
• In addition, there is another potential splice form that shows a 16aa substitution for aa1281 fulllength human EGLN2 shares 91% aa sequence identity with mouse EGLN2.
• Applications:Suitable for use in Western Blot and Immunoprecipitation.
• Recommended Dilution:Western Blot: 1ug/mlImmunoprecipitation: 25ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.