PACAP (Pituitary adenylate cyclase activating polypeptide) is a 17kD (predicted) member of the VIP/secretin/glucagon/GHRH superfamily of peptides.
It is expressed by anterior pituitary gonadotrophs, neurons in the CNS and PNS, spermatids, adrenal chromaffin cells, plus macrophages and lymphocytes.
PACAP has multiple effects.
On mast cells, it induces histamine release; on lymphocytes, PACAP promotes Th2 development; in bone, PACAP inhibits osteoclastogenesis; and in the pituitary, PACAP facilitates the release of LH (in females), GH and a-MSH.
Mouse PACAP proprecursor is 151aa in length (aa25-175).
It undergoes proteolytic processing to generate two C-terminal amidated peptides termed PACAP-38 (aa131-168) and PACAP-27 (aa131-157).
In general, PACAP-38 represents the dominant form.
However, the ratio between the -38 and -27 forms differs in a cell-specific manner.
Over (aa25-175), mouse PACAP shares 95% and 81% aa identity with rat and human PACAP, respectively.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Direct ELISA: 0.5-1ug/mlWestern Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with sterile PBS.
Specificity：Recognizes mouse PACAP.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo
Immunogen：Recombinant corresponding to mouse PACAP expressed in E. coli (070176).
