84-1190-20　Notch3 (Notch Homolog 3 (Drosophila), hN3, Neurogenic Locus Notch Homolog Protein 3, CADASIL, CASIL) (APC) 100Tests　N5375-26E

特徴

• Human Notch-3 is part of the Notch family of type I transmembrane glycoproteins involved in a number of early event developmental processes.1 The extracellular domains of Notch receptors interact with the extracellular domains of the transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of a neighboring cell.
• In both vertebrates and invertebrates, Notch signaling is important for specifying cell fates and for defining boundaries between different cell types.
• Notch-3 has the same biochemical mechanism of signal transduction as Notch-1, where a series of cleavage events release of the Notch intracellular domain (NICD).
• NICD translocates to the nucleus and initiates transcription of Notch responsive genes.2 Thus, Notch acts as both a ligand binding receptor and a nuclear factor that regulates transcription.
• Human Notch-3 shows 90% aa identity to mouse Notch-3 over the entire protein.
• Applications:Suitable for use in Flow Cytometry.
• Other applications not tested.
• Recommended Dilutions:Flow Cytometry: 10ul to label 1x10e6 cellsOptimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: APC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.