MUC-1 (Mucin-1; also episialin, MAM-6, PEM and PUM) is a 150-450kD type I transmembrane glycoprotein that belongs to the Mucin family of molecules.
It forms a protective barrier against microbes on the apical surface of most glandular epithelium, and becomes prominently displayed on tumor cells.
Mature human MUC-1 is 1232aa in length.
It contains an 1135aa extracellular domain (ECD) (aa24-1158), and a signal transducing 73aa cytoplasmic tail (aa1182-1255).
The ECD is highly polymorphic as evidenced by a highly variable number of 20 aa O-glycosylated tandem repeats.
Intracellular processing cleaves MUC-1 between Gly1097 and Ser1098, generating a noncovalently linked heterodimer that is expressed on the cell surface.
The C-terminus is 22-25kD in size, while the N-terminus is highly variable (70-400kD in size), a function of the number of resident tandem repeats.
Cell surface processing by TACE releases a soluble ECD fragment of variable MW.
There are multiple splice variants that show massive reductions in the size of the ECD.
Over (aa126-145), human MUC-1 shares < 50% aa identity with mouse MUC-1.
When compared to human, mouse MUC-1 is found not to be polymorphic and possess poorly conserved tandem repeat sequences.
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: 5-15ug/ml using immersion fixed paraffin-embedded sections of human breastWestern Blot: 1ug/ml using human lung tissueOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human MUC-1. Crossreactivity: <5% recombinant human MUC-16.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Pro126-Arg145 from human MUC-1, expressed in E. coli. Uniprot: P15941.
