LAG-3, also known as CD223, is a type I membrane protein that belongs to the immunoglobulin superfamily (IGSF).
The extracellular domain of LAG-3 contains a V-set and three C2-set Ig domains.
LAG-3 is expressed on activated T cells, NK cells and B cells.
It binds to MHC class II molecules with much higher avidity than CD4, a LAG-3 structural homolog.
Mouse and human LAG-3 have been shown to be negative regulators of T cell function and homeostasis.
The extracellular domain of human and mouse LAG-3 share 69% aa sequence identity.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 10ul labels 1x10e6 human peripheral blood mononuclear cells (PBMCs) treated with PHAOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100Tests
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography.
Form：Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Specificity：Recognizes human LAG-3. In direct ELISA and Western Blot, <1% crossreactivity is observed with recombinant mouse LAG-3.
Isotype：IgG
Calc Applications Abbrev：FC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Leu23-Leu450 of human LAG-3, expressed in NS0 cells. Swiss/UniProt Accession: P18627
