84-1190-13　Leucyl-cystinyl Aminopeptidase/LNPEP 100ug　I8601-01C

特徴

• LNPEP (also known as PLAP, CAP, IRAP, and Oxytocinase) is a 165-175kD member of the oxytocinase subfamily, M1 family of zinc metallopeptidases.
• It is widely expressed after cell stimulation and is found on endothelial cells, trophoblasts, skeletal muscle cells, neurons, adipocytes, and renal distal tubule epithelium.
• LNPEP cleaves vasopressin and oxytocin and serves as a receptor for AngIV.
• Human LNPEP is a 1025aa type II transmembrane glycoprotein
• It contains a 110aa cytoplasmic region (aa1110)plus an 894aa extracellular domain (aa1321025) that contains a peptidase M1 region(aa168552).There are multiple potential splice variants.
• There are two alternative start sites at Met15 and Met20, and the cytoplasmic tail undergoes phosphorylation at Tyr70 and Ser80.
• LNPEP is reportedly forms homodimers.
• Proteolytic cleavage between Phe154Ala155 generates a 150155kD soluble form.
• Over (aa 133552), human LNPEP shares 89% aa sequence identity with mouse LNPEP.
• Applications:Suitable for use in Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlImmunoprecipitation: 25ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.