Two type I membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with high affinity.
The lower affinity IL-13 binding protein is now referred to as IL-13 Receptor, alpha 1 and is also known as CD213a.
IL-13 Receptor alpha 1 combines with IL-4 Receptor alpha to form a high affinity receptor complex capable of transducing an IL-13-dependent proliferative signal.
The higher affinity IL-13 binding protein, now referred to as IL-13 Receptor, alpha 2, does not induce a signal and acts as a decoy receptor.
Cell surface expression of IL-13 Receptor, alpha 2 is determined by flow cytometry using 620-650nm wavelength excitation and monitoring emitted fluorescence with a detector optimized to collect peak emissions at 660-670nm.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 10ul labels up to 1x10e6 cells.
Validated for use with A375 human melanoma cell lineOptimal dilutions to be determined by the researcher.
Flow Cytometry Protocol:1.
Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT.
Do not wash excess blocking IgG from this reaction
2.
After blocking, add 10ul of I8435-30A to up to 1x10e6 cells and incubate for 30 minutes at RT
3.
Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer (F4568-10).
Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer
4.
Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis.
As a control for this analysis, cells in a separate tube should be treated with APC-labeled goat IgG antibody.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100Tests
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Specificity：Recognizes human Interleukin 13 Receptor, alpha 2. Shows ~5% crossreactivity with recombinant mouse Interleukin 13 Receptor, alpha 2.
Isotype：IgG
Calc Applications Abbrev：FC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Cys22-Leu342 of human Interleukin 13 Receptor, alpha 2 expressed in NS0 cells.
