84-1190-10　IRF1 (Interferon Regulatory Factor 1, IRF-1, MAR) 100ug　I7662-81G

特徴

• IRF1(interferon regulatory factor1) is a 45kD member of the IRF family of proteins.
• It is induced by TLR9 and INF stimulation, and positively regulates theexpression of IFNb NOS and1L12p35
• Human IRF1 is 325aa in length.
• It contains an Nterminal DNA binding basic region (aa1140)and an acidic Cterminus(aa147-325).
• Lys275 and 299 can be SUMOylated, which blocks activity, or ubiquinated at Lys299, which initiates degradation.
• There is a splice variant that shows an 11aa substitution for the C terminal 86aa.
• Over aa171-325,human IRF1 shares 83% aa identity with both canine and porcine IRF1.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.