IFN-a/b R2 belongs to the type II cytokine receptor family.
It complexes with IFN-a/b R1 to form the signaling receptor complex for the family of a and IFN subtypes.
By alternative splicing, IFN-a/b R2 can exist as a secreted soluble protein or as a type I membrane protein.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 10ul/10e6 cells using U937 human histiocytic lymphoma cell line
Optimal dilutions to be determined by the researcher.
Flow Cytometry ValidationThis antibody has been tested for Flow Cytometry using A20 cells
1.
Cells may be Fc-blocked with 1ug of mouse IgG/10e5 cells for 15 minutes at RT.
Do not wash excess blocking IgG from this reaction
2.
After blocking, 10ul I7661-59C was added to 1-2.5 x 10e5 cells and incubated for 30 minutes at RT
3.
Unbound antibody was removed by washing the cells twice in F4568-10 Flow Cytometry Staining Buffer.
Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Mouse Lyse Buffer
4.
The cells were resuspended in F4568-10 Flow Cytometry Staining Buffer for final flow cytometric analysis.
As a control for this analysis, cells in a separate tube should be treated with CFS-labeled goat IgG antibody.
This procedure may need to be modified, depending upon the cell type and final utilization.
Individual users may need to titrate to determine the optimal reagent amount for their specific use
5.
Cell surface expression of IFN-a/b R2 was determined by flow cytometry using 488nm wavelength excitation and monitoring emitted fluorescence with a detector optimized to collect peak emissions at 515-545nm.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Carboxyfluorescein conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100Tests
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in saline containing up to 0.5% BSA, 0.09% sodium azide. Labeled with Carboxyfluorescein (CFS).
Specificity：Recognizes mouse IFN-alpha/beta receptor 2. Species Crossreactivity: human. In Western blots, ~10% crossreactivity with recombinant mouse IFN-g R2 and rmIL-20Ra was observed and ~5% crossreactivity with recombinant human IL-10Rb, rmIFN-g RI and rhIL-20Rb was observed and <1% crossreactivity with rhIFN-b Ra was observed.
Isotype：IgG
Calc Applications Abbrev：FC
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Ser22-Ala239 (Lys160Asn) from human IFNa/b R2 expressed in NS0 cells. UniProt Accession: Q9D1R7.
