IGFI receptor is a disulfidelinked heterotetrameric transmembrane protein consisting of two a and two b subunits.
Both the a and b subunits are encoded within a single receptor precursor cDNA.
The proreceptor polypeptide is proteolytically cleaved and disulfidelinked to yield the mature heterotetrameric receptor.
The a subunit of IGFI receptor is extracellular while the b subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain.
The IGFI receptor is highly expressed in all cell types and tissues.
Essentially all of the biological activities of IGFI and II have been shown to be mediated via IGFI R.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Neutralization and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1ug/mlImmunohistochemistry (paraffin): 5-15ug/ml using immersion fixed paraffin-embedded sections of human placenta (chorionic villi) and immersion fixed frozen sections of mouse embryoNeutralization: 0.5-1.5mg/ml in the presence of 6ng/ml recombinant human IGF-I
Immunocytochemistry:1-15ug/ml using immersion fixed MCF-7 human breast cancer cell line
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography. Endotoxin: ≤0.1EU/1mg (LAL).
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human Insulin-Like Growth Factor I Receptor. Species crossreactivity: mouse. In direct ELISAs and Western blots, 25-50% cross-reactivity with recombinant mouse IGF-I R is observed. In direct ELISAs, less than 1% cross-reactivity with recombinant human IGF‑II R is observed.
Isotype：IgG
Calc Applications Abbrev：IC IHC N WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to the extracellular domain of human Insulin-Like Growth Factor I Receptor.
