Heparan Sulfate is a highly sulfated polysaccharide that can be found on cell surface and extrracellular matrix.It is usually covalently attached to a protein core as the glycan component of a proteoglycan.
Heparan Sulfate interacts with a variety of proteins,such as growth factors, protease inhibitors, cytokines, lipoprotein lipase and viral envelope proteins, thus plays roles from cell growth, cell differentiation, cell motility, blood coagulation, lipid metabolism to viral infection (1,2).Heparan Sulfate consists of repeating residues of uronic acid and N-acetyl glucosamine.
The uronic acid residues can be sulfated at 2-O position by Heparan Sulfate 2-0 Sulfotransferase (HS2ST).
The N-acetyl glucosamine residues can be sulfated at N-, 3-O, and 6-O positions by N-deacetylase/N-sulfotransferases (NDSTs), Heparan Sulfate 3-O and 6-O sulfotransferases (HS3STs and HS6STs) respectively.
However, the reactions catalyzed by these sulfotransferases are normally incomplete on the whole chain of Heparan Sulfate.
As a result, Heparan Sulfate displays enormous sequence diversity that allows it to interact with a wide spect rum of proteins differently.
Among three HS6STs, HS6ST1is the first to be cloned(3).Mice deficient of the HS6ST1 homologue gene showed embryonic lethality(4).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1mg/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, with Trehalose. Reconstitute with sterile PBS.
Specificity：Recognizes human.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to human HS6ST1 expressed in CHO cells (AAY14736).
