FOLR4 (Folate receptor 4; also FR4 and folate receptor dH2O and folate binding protein 3) is a 34-36kD member of the folate receptor family of molecules.
Based on mouse, it appears to be a marker for subsets of T cells.
In particular, and in conjunction with CD25, FR4hiCD25hi expression defines Treg cells, FR4loCD25+ expression pattern identifies effector memory T cells, and a FR4hiCD25- combination characterizes central memory T cells.
Mature human FOLR4 is 225aa in length (aa20-244).
It contains virtually no identifiable motifs.
There is one potential splice variant that shows a five aa substitution for (aa111-116).
Over (aa134-220) human FOLR4 shares 72% aa identity with mouse FOLR4.
Applications:Suitable for use in Direct ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml using A431 human epithelial carcinoma cell lineOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS
Specificity：Recognizes human FOLR4. Shows >5% cross-reactivity with recombinant human FOLR1, FOLR2 and FOLR3 in direct ELISA.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Glu134-Asn210 from human FOLR4, expressed in E. coli.
