CXCR5 (CXC chemokine receptor 5; also BLR-1, NLR and CD185) is a 55-60kD member of the G-protein coupled receptor 1 family.
It is expressed on select cell types, including granule and Purkinje cell neurons, embryonic CD4+ CD3- IL-7Ra precursor cells, B cells and follicular T helper cells.
CXCR5 selectively binds BLC/CXCL13.
This appears to both promote embryonic lymph node development and, in the adult, direct expressing cells to positions that optimize antigen presentation and antibody production.
Mouse CXCR5 is a 7-transmembrane glycoprotein thatis 374(aa) in length.
It contains a 57 aa N-terminal extracellular region plus a 47aa C-terminal cytoplasmic domain.
Over aa1-57, mouse CXCR5 shares 84% and 47% aa identity with rat and human CXCR5, respectively.
Applications:Suitable for use in Flow Cytometry and Direct ELISA.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse CXCR5. Shows <1% crossreactivity with mouse CXCR7 in direct ELISA.
Isotype：IgG
Calc Applications Abbrev：E FC
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Met1-Pro57, Glu112-Thr126, Lys191-Phe221, and Leu283-Ile306 from mouse CXCR5 joined by linker peptides and expressed in E. coli.
