Recommended Dilutions:Western Blot: 0.1-0.2 ug/ml The detection limit for recombinant Clostridium botulinum Neurotoxin Type D Light Chain is ~1ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile TBS, 0.1% BSA.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months after receipt at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:50ug
Host:sheep
Source Antibody:E. coli
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder from PBS, 50ug BSA. Labeled with Biotin. Reconstitute with 1ml sterile TBS, 0.1% BSA.
Specificity:Recognizes BoNT-D Light Chain.
Isotype:IgG
Calc Applications Abbrev:WB
Calc Crossreactivity:Ec
Immunogen:Recombinant corresponding to aa2-428 from BoNT-D Light Chain expressed in E. coli (P19321).