Recommended Dilutions:Western Blot: 0.1ug/ml using recombinant Botulinum Neurotoxin Type A Light Chain Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:50ug
Host:sheep
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography
Form:Supplied as a yophilized powdef from PBS, pH 7.4, BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS
Specificity:Recognizes C. botulinum BoNT-A Light Chain. In Western blot, exhibits <1% cross-reactivity with recombinant BoNT-A heavy chain receptor.
Isotype:IgG
Calc Applications Abbrev:WB
Calc Crossreactivity:Ec
Immunogen:Recombinant protein corresponding to Met1-Phe425 of Botulinum Neurotoxin Type A light chain, expressed in E. coli