84-1189-88　CCCTC-Binding Factor (CTCF, CTCFL Paralog, 11-Zinc Finger Protein) 100ug　C2099-05G

特徴

• CTCF (CCCTC-binding factor) is a 130-140kD member of the CTCF Zn-finger protein family.
• Although its predicted MW is 83kD, it runs anomalously slow in SDS-PAGE.
• CTCF is ubiquitously expressed, save for primary spermatocytes, and serves multiple functions.
• It regulates both gene activation and repression by binding to unmethylated DNA sequences.
• Structurally, it contains eleven Zn-finger motifs, some or all of which may be involved in DNA binding.
• This variation in number allows for different conformations and multiple interaction partners.
• Human CTCF is 727 aa in length.
• It contains two SUMOylation sites (Lys74 and Lys689) and eleven consecutive C2H2-type Zn-finger domains (aa266-577).
• There are multiple Ser/Thr phosphorylation sites.
• Two potential alternative start sites exist; one nine aa upstream of thestandard start site, and another at Met570.
• A third splice variant shows a 62aa substitution for (aa349-727).
• Over (aa1-154), human CTCF shares 99% aa identity with mouse CTCF.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/ml using MOLT-4 human acute lymphoblastic leukemia cell line and Jurkat human acute T cell leukemia cell line
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.