ARHGAP1(also p50-RhoGAP and CDC42 GTPase-activating protein) is a 50kD member of the ARHGAP family of proteins.
It is preferentially expressed in epithelium, and serves as a cytoplasmic protein that deactivates Rho-family GTPases.
It does so by contributing an Arg residue (Arg282)to Cdc42 and RhoA, which activates their intrinsic GTPase activity and converts GTP-associated molecules into inactivating GDP molecules.
Human ARHGAP1 is 439aa in length and contains one CRAL-TRIO domain that binds small lipophilic molecules (aa53-218), an SH3 binding site (aa228-238) and one C-terminal RhoGAP domain (aa244-431).
There are phosphorylation sites at Ser51 and Tyr65.
There is one potential splice form with a 24aa substitution for (aa1-150)coupled to a 26aa substitution for (aa300-439), Over (aa1-62, human ARHGAP1 shares 96% aa identity with mouse ARHGAP1.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with sterile PBS.
Specificity：Recognizes human, mouse and rat ARHGAP1.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa1-62 from human ARHGAP1 expressed in E. coli (Q07960).
