Osteocrin is a recently identified secreted protein expression of which was only detected in bone, peaking just after birth and decreasing markedly with age.
A 1280-bp mRNA encodes osteocrin producing a mature protein of 103 amino acids with a molecular mass of 11.4kD.
In primary osteoblastic cell cultures osteocrin expression coincided with matrix formation then decreased in very mature cultures.
Treatment of cultures with 1,25-dihydroxyvitamin D3 resulted in a rapid dose-dependent down-regulation of osteocrin expression, suggesting direct regulation.
Chronic treatment of primary cultures with osteocrin-conditioned media inhibited mineralization and reduced osteocalcin and alkaline phosphatase expression.
These results suggest that osteocrin represents a novel, unique vitamin D-regulated bone-specific protein that appears to act as a soluble osteoblast regulator.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:12,800Western Blot: 1:100Optimal dilutions to be determined by the researcher.
AA Sequence:Ser-Phe-Ser-Gly-Phe-Gly-Ser-Pro-Leu-Asp-Arg-Leu-Ser-Ala-Gly-Ser-Val-Glu-His-Arg-Gly-Lys-Gln
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months after receipt at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：200ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a lyophilized powder from 60ul of PBS. Reconstitute with 200ul sterile PBS.
Specificity：Recognizes mouse Musclin. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo Rt
Immunogen：Synthetic peptide corresponding to aa80-102 of mouse Musclin and aa82-104 of rat Musclin.
