Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Indirect ELISA: 1:12800Immunohistochemistry (formalin-fixed paraffin): 1:500-1000 (ABC, DAB)Western Blot: 1:500-1000Optimal dilutions to be determined by the researcher.
Positive Control:Mouse and rat visceral white adipose tissue homogenate
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ul
Host:rabbit
Source Antibody:rat
Grade:Serum
Purity:Serum
Form:Supplied as a lyophilized powder. Reconstitute with sterile dH2O.
Specificity:Recognizes rat Leptin. Species crossreactivity: mouse.
Isotype:IgG
Calc Applications Abbrev:E IHC WB
Calc Crossreactivity:Mo Rt
Immunogen:Recombinant peptide corresponding to rat Leptin.