Glucagon is a member of a multigene family comprising of Secretin, Vasoactive Intestinal Peptide (VIP), Gastric Inhibitory Peptide (GIP) and others like Glicentin and Oxyntomodulin (OXM), which differs from glucagon by C-terminal octapeptide.
The glucagon precursor contains at least 3 intervening sequences that divide the protein-coding portion into 4 regions corresponding to the signal peptide and part of the N-terminal peptide, the remainder of the N-terminal peptide and glucagon, glucagon-like peptide-1 (GLP1), and GLP2.
The GLP 1 & 2 stimulates intestinal growth and up regulates villus height in the small intestine, concomitant with increased crupt cell proliferation and decreased enterocyte apoptosis.
The two GLP's are mainly produced in the A cells of the Islets of Langerhans in response to a drop in blood sugar concentration.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilutions:ELISA: 1:12,800 (0.0781ug/ml)Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O/PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：400ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography from whole serum.
Form：Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 302ul of dH2O, then add 98ul of sterile PBS.
Specificity：Recognizes human GLP-1. Species Crossreactivity: mouse and rat GLP-1 (7-37). Does not react with human GLP-1 (aa7-36), human GLP-2, human Glucagon or human Insulin.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa7-37 of human GLP-1.
