Gastric inhibitory polypeptide (GIP; MIM 137240), also called glucose-dependent insulinotropic polypeptide, is a 42 amino acid polypeptide synthesized by K cells of the duodenum and small intestine.
It was originally identified as an activity in gut extracts that inhibited gastric acid secretion and gastrin release, but subsequently was demonstrated to stimulate insulin release potently in the presence of elevated glucose.
The insulinotropic effect on pancreatic islet beta-cells was then recognized to be the principal physiologic action of GIP.
Together with glucagon-like peptide-1, GIP is largely responsible for the secretion of insulin after eating.
It is involved in several other facets of the anabolic response.
Applications:Suitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:128,000Optimal dilutions to be determined by the researcher.
AA Sequence:Tyr-Ala-Glu-Gly-Thr-Phe-Ile-Ser-Asp-Tyr-Ser-Ile-Ala-Met-Asp-Lys-Ile-His-Gln-Gln-Asp-Phe-Val-Asn-Trp-Leu-Leu-Ala-Gln-Lys-Gly-Lys-Lys-Asn-Asp-Trp-Lys-His-Asn-Ile-Thr-Gln
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute with 50ul sterile ddH2O.
Specificity：Recognizes human GIP. Species Crossreactivity: Porcine (100%), rat (31%). Does not crossreact with human Gastrin I, human Big Gastrin I, human Gastrin Releasing Peptide, Bombesin, Substance P or human Insulin.
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu Po Rt
Immunogen：Synthetic peptide corresponding to human GIP.
