Applications:Suitable for use in Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:200Immunohistochemistry (formalin-fixed paraffin): 1:200 (ABC, DAB)Optimal dilutions to be determined by the researcher.
Positive Control:Rat kidney and mouse lung tissue
AA Sequence:Cys-Ser-Cys-Ser-Ser-Leu-Met-Asp-Lys-Glu-Cys-Val-Tyr-Phe-Cys-His-Leu-Asp-Ile-Ile-Trp
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ul
Host：rabbit
Source Antibody：rabbit
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute with sterile dH2O.
Specificity：Recognizes rabbit Endothelin-1. Species crossreactivity: Endothelin-1 (mouse, porcine, canine, rat, bovine). % Cross-reactivity with Peptides: Endothelin-1 (Human, Rat, Mouse, Porcine, Bovine, Canine, Rabbit, Monkey): 100%; Big Endothelin-2 (Human): 70%; Big Endothelin-1(Human): 50%; Endothelin-3 (Human, Rat, Porcine): 28%; Endothelin-2 (Human, Canine): 3.5%; Big Endothelin-3 (Human): 0.2%; Angiotensin II (Human): 0%; [Arg8]-Vasopressin: 0%
Isotype：IgG
Calc Applications Abbrev：IF IHC
Calc Crossreactivity：Bo Ca Hu Mo Po Rb Rt
Immunogen：Synthetic peptide corresonds to rabbit Endothelin-1.
