Applications:Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:200Immunocytochemistry: 1:200Immunohistochemistry (Paraffin): 1:2000 (ABC, DAB)Optimal dilutions to be determined by the researcher.
Positive Control:Rat Kidney tissue
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:50ul
Host:rabbit
Source Antibody:human
Grade:Serum
Purity:Serum
Form:Supplied as a lyophilized powder. Reconstitute with 50ul sterile dH2O.
Specificity:Recognizes human Angiotensin II. Species Crossreactivity: rat, mouse and canine. Expected to cross-react with porcine, rabbit and guinea pig based on sequence homology. Crossreactivity: Angiotensin III (human): 100%; Angiotensin I (human): 1.7%; Pre-Angiotensin (1-14): 1%; Angiotensin II (1-4) (human): 0.1%; Angiotensin II (5-8) (human): 0.001%; ACTH (human): 0%; Endothelin-1 (human, porcine, rat): 0%; [Arg8]-Vasopressin: 0%
Isotype:IgG
Calc Applications Abbrev:IC IHC WB
Calc Crossreactivity:Ca Hu Mo Rt
Immunogen:Synthetic peptide corresponding to DRVYIHPF from human Angiotensin II (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe).