Adiponutrin is one of three recently identified adipocyte lipases.
Surprisingly, these proteins also retain transacylase activity, a hitherto unknown pathway of triacylglycerol synthesis in the adipocytes.
This may enable them to participate in both anabolic and catabolic processes.
The adiponutrin gene (ADPN) is downregulated by fasting and upregulated by refeeding, suggesting a role in lipogenesis.
Experiments in human adipocytes confirmed that the gene is upregulated in response to insulin in a glucose-dependent fashion.
Obese subjects had increased levels of subcutaneous and visceral abdominal adipose tissue ADPN mRNA.
Visceral ADPN mRNA expression was correlated to measures of insulin sensitivity (fasting insulin and homeostasis model assessment).
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:ELISA: 1:6400, 0.156ug/mlOptimal dilutions to be determined by the researcher.
AA Sequence:Cys-Pro-Ala-Glu-Thr-Lys-Ala-Glu-Ala-Thr-Pro-Arg-Ser-Ile-Leu-Arg-Ser-Ser-Leu-Asn-Phe-Phe-Leu-Gly-Asn-Lys-Val-Pro-Ala-Gly-Ala-Glu-Gly-Leu-Ser-Thr-Phe-Pro-Ser-Phe-Ser-Leu-Glu-Lys-Ser-Leu
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：200ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder from PBS. Reconstitute with 200ul of sterile PBS.
Specificity：Recognizes human Adiponutrin.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa436-481 of human Adiponutrin.
