Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action (1,2).
Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD, and JAMM enzymes.
USP10 possessesaa sequences that match the consensus cysteine and histidine boxes representative of the USP family of deubiquitinating enzymes.
At the posttranslational level, USP10 appears to be regulated through both protein-protein interactions and phosphorylation.
Indeed, interaction of USP10 with Ras-GAP SH3 domain binding protein (G3BP) has been found to inhibit its ability to catalyze the disassembly of ubiquitin chains (3).
Furthermore, ATM-mediated phosphorylation of USP10 at Thr42 and Ser337 was shown to promote its stabilization and redistribution from the cytoplasm to the nucleus, where it functions in p53 deubiquitination, stabilization, and activation in response to genotoxic stress (4).
USP10 also functions in the endosomal compartment, where it has been shown to deubiquitinate CFTR in order to enhance its endocytic recycling and cell surface expression (5,6).
Applications:Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Immunofluorescence (IF-IC): 1:50Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Immunoprecipitation: 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of total human USP10. Species Crossreactivity: monkey, mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IF IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to residues surrounding Leu163 of human USP10.
