Focal adhesions connect the cytoskeleton with the extracellular matrix (ECM), a complex structure of secreted macromolecules that surrounds mammalian organs and tissues.
Integrins clustered on the extracellular side of focal adhesions signal from the ECM to intracellular proteincomplexes, which in turn signal to the actin cytoskeleton to regulate the tension needed for cell motility.
Internal signals also converge on focal adhesions to regulate integrin affinity and avidity.
Signaling through focal adhesions regulates cell adhesion, migration, proliferation, apoptosis andgene expression, and impacts cellular processes such as development, wound healing, immune response, invasion, metastasis and angiogenesis (reviewed in 1-3).
Talin is a large, multidomain focal adhesion protein that interacts with the intracellular domains of integrins and other focaladhesion proteins.
Talin is involved in the formation of focal adhesions and in linking focal adhesions to the actin cytoskeleton (4).
The interaction between talin and integrins increases the affinity between integrin and both insoluble and soluble ECM proteins (5,6).
Phosphorylation of talin at Ser425 was discovered using mass spectrometry.
This site is a potential substrate of CDK5 (7).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of human talin protein only when phosphorylated at Ser425. Species Crossreactivity: monkey, mouse, rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to residues surrounding Ser425 of human talin.
