Protein phosphatase 1 (PP1) is a ubiquitous eukaryotic protein serine/threonine phosphatase involved in the regulation of various cell functions.
Substrate specificity is determined by the binding of a regulatory subunit to the PP1 catalytic subunit (PP1c).
It is estimated that over fifty different regulatory subunits exist.
The myosin phosphatase holoenzyme is composed of three subunits: the PP1 catalytic subunit (PP1c), a targeting/regulatory subunit (MYPT/myosin-binding subunit of myosin phosphatase) and a 20kD subunit of unknown function (M20).
MYPT binding to PP1cd alters the conformation of the catalytic cleft and increases enzyme activity and specificity.
Two MYPT isoforms that are 61% identical have been described.
MYPT1 is widely expressed while MYPT2 expression appears to be exclusive to heart and brain.
Related family members also include MBS85, MYPT3 and TIMAP.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of human MYPT1 at ~140kD only when phosphorylated at threonine 696. Species Crossreactivity: monkey, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues surroundiing Thr696 of human MYPT1.
