HMGA2 belongs to the family of high mobility group with AT-hook DNA binding domain.
HMGA proteins are considered architectural transcription factors; they do not have direct transcriptional activation capacity, but instead regulate gene expression by changing DNA conformation through binding to AT-rich regions in the DNA, and/or direct interaction with other transcription factors (1,2).
HMGA2 is abundantly and ubiquitously expressed and plays a crucial role during embryonic development (3).
HMGA2 promotes stem cell self-renewal and decreases in its expression are associated with stem cell aging (4-7).
Expression levels of HMGA2 are very low in normal adult tissues, but its overexpression or rearrangement is associated with many types of cancer (8-11).
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Immunoprecipitation: 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of total human HMGA2. Species Crossreactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues near the N-terminus of human HMGA2.
