Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:200-1:5000Western Blot: 1:200-1:5000Immunohistochemistry: 1:500-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Further dilutions can be made in assay buffer.
Labeled with horseradish peroxidase (HRP).
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:500ug
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography (≥90% SDS-PAGE)
Form:Supplied as a lyophilized powder from PBS, pH 7.2, 0.1% BSA (Protease/IgG free), 0.1% Kathon CG. Labeled with horseradish peroxidase (HRP). Reconstitute with 500ul sterile ddH2O.
Specificity:Recognizes the heavy (mu) chains on human IgM based on IEP. Does not recognize non-immunoglobulin human serum proteins or human IgA or IgG.