Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:50,000Western Blot: 1:10,000-1:50,000Immunohistochemistry: 1:500-1:5000 Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder is stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.2, 0.1% proclin 150, 1% BSA (Protease/IgG free), 0.1% Kathon CG. Reconstitute with 1.1 ml ddH2O and let stand 30 minutes at room temperature to dissolve. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes heavy (u) chains on human IgM. Does not recognize non-immunoglobulin human serum proteins, light chains on all human immunoglobulins, human IgA or IgG.
