Applications:Suitable for use in FLISA, Flow Cytometry, Immunohistochemistry, Immunocytochemistry and Immunomicroscopy.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:20-1:2000 Immunocytochemistry: 1:50-1:5000 Optimal dilutions to be determined by the researcher.
Absorption:494nm
Emission:518nm
FITC/Protein Ratio:7.2 Moles FITC per Mole Antibody
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:2mg
Host:rabbit
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in 10mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 1% BSA, Protease/IgG free, 0.05% sodium azide. labeled with Fuorescein Isothiocyanate (FITC).
Specificity:Recognizes heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on IEP. No reactivity is obserced to non-immunoglobulin mouse serum proteins.