Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:200-1:5000Western Blot: 1:200-1:5000Immunohistochemistry: 1:500-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder is stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：goat
Source Antibody：rabbit
Grade：Affinity Purified
Purity：Purified by affinity chromatography
Form：Supplied as a lyophilized powder from 10mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 1% BSA (Protease and IgG free), 0.1% ProClin-150. Reconstitute with 550ul of sterile ddH2O. Labeled with Horseradish peroxidase (HRP).
Specificity：Recognizes heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins based on IEP.
