Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:200-1:5,000Western Blot: 1:200-1:5,000Immunohistochemistry: 1:500-1:5000 Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder is stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography. ~90% by SDS-PAGE. May contain small amounts of intact IgG.
Form：Supplied as a lyophilized powder from 10mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 1% BSA (Protease/IgG free), 0.1% Kathon CG. Labeled with HRP. Reconstitute with 550ul sterile ddH2O.
Specificity：Recognizes heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on IEP. Does not react with non-immunoglobulin mouse serum proteins.
