Applications:Suitable for use in ELISA, Western Blot,Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:500-1:2000Western Blot: 1:500-1:2000Immunohistochemistry: 1:20-1:2000 Immunocytochemistry: 1:50-1:5000 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C before opening.
DO NOT FREEZE! Stable at 4°C as an undiluted liquid.
Dilute only prior to immediate use.
Stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
仕様
Size:500ug
Host:goat
Source Antibody:mouse
Grade:Affinity Purified
Purity:~90% based on SDS-PAGE, small amounts of intact IgG may be present.
Form:Supplied as a liquid in 30mM triethanolamine, pH 7.2, 5mM magnesium chloride, 0.1mM zincchloride, 1% BSA, Protease/IgG free, 0.05% sdium azide. Labeled with Alkaline Phosphatase (AP).
Specificity:Recognizes heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on IEP.