Applications:Suitable for use in Flow Cytometry, Immunohistochemistry, Immunocytochemistry and Immunomicroscopy.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:20-1:2000Immunocytochemistry: 1:50-1:5000Optimal dilutions to be determined by the researcher.
Absorption: 494nm
Emission: 518nm
FITC/Protein Ratio: 3-7 Moles FITC per Mole Antibody
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
FITC conjugates are sensitive to light.
仕様
Size：500ug
Host：goat
Source Antibody：rat
Grade：Affinity Purified
Purity：~90% based on SDS-PAGE, small amounts of intact IgG may be present.
Form：Supplied as a liquid in 10mM sodium phosphate, 0.15M sodium chloride, pH7.2, 1% BSA, Protease/IgG free, 0.05% sodium azide. Labeled with Fuorescein Isothiocyanate (FITC).
Specificity：Recognizes heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on IEP. Does not recognize non-immunoglobulin rat serum proteins or IgG from human or mouse.
