Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:5000-1:50,000; Gives an A450 reading of ≥ 1.0 when tested against rabbit IgG (H&L) coated at 2ug/ml
Immunohistochemistry: 1:500-1:5000Western Blot: 1:10,000-1:50,000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Labeled with horseradish peroxidase (HRP).
Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
HRP conjugates are sensitive to light.
仕様
Size：500ug
Host：chicken
Source Antibody：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 1% BSA (Protease/IgG free), 0.1% Kathon CG. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on IEP. Bands are observed at 24kD and 60kD based on SDS-PAGE. Does not crossreact with non-immunoglobulin rabbit serum proteins, human IgG or mouse IgG.
