Applications:Suitable for use in Flow Cytometry, Immunohistochemistry, Immunocytochemistry and Immunomicroscopy.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:20-1:2000 Immunocytochemistry: 1:50-1:5000 Optimal dilutions to be determined by the researcher.
Absorption: 494nm
Emission: 518nm
FITC/Protein Ratio: 3-7 Moles FITC per Mole Antibody
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
FITC conjugates are sensitive to light.
仕様
Size：500ug
Host：rabbit
Source Antibody：goat
Grade：Affinity Purified
Purity：~90% based on SDS-PAGE, may contain small amounts of intact IgG.
Form：Supplied as a liquid in 10mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 1% BSA, Protease/IgG free, 0.05% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on IEP. Does not recognize non-immunoglobulin goat serum immunoglobulins. Does not recognize IgG from human, mouse or rat.
