Applications:Suitable for use in FLISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry and Immunomicroscopy.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: 1:20-1:2000Immunocytochemistry: 1:50-1:5000Optimal dilutions to be determined by the researcher.
Absorption:494nm
Emission:518nm
FITC/Protein Ratio:7 Moles FITC per Mole Antibody
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography. ≥95% by SDS-PAGE.
Form：Supplied as a liquid in 10mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 1% BSA (Protease and IgG free), 0.05% sodium azide. Labeled with Fuorescein Isothiocyanate (FITC).
Specificity：Recognizes epsilon chains on human IgE based on IEP. No reactivity is observed with light chains on human immunoglobulins and on non-immunoglobulin human serum proteins.
