84-1176-37　Zinc Transporter 7 (ZIP7, ZnT-7, Solute Carrier Family 30 Member 7, Znt-like Transporter 2, SLC30A7, ZNT7, ZNTL2) 100ul　Z0510-09

特徴

• Zinc is an essential nutrient for all organisms because of the many important roles this metal plays.
• Movement of zinc into and out of cells and subcellular organelles is mediated by zinc transporter proteins.
• In many organisms, zinc uptake is mediated by members of the ZIP family of metal ion transporters.
• In mammals, the Zip1, Zip2, ZIP4, Zip4, ZIP7, LIV-1 (ZIP7), KE4 (Zip7), and BIGM103 (Zip8) proteins have been implicated in zinc uptake in a variety of cell and tissue types.
• ZIP7 (Ke4, Slc39a7) also belongs to the ZIP family of zinc transporters.
• Transient expression of the V5-tagged human ZIP7 fusion protein in CHO cells led to elevation of the cytoplasmic zinc level.
• However, the precise function of ZIP7 in cellular zinc homeostasis is not clear.
• Here we report that the ZIP7 gene is ubiquitously expressed in human and mouse tissues.
• The endogenous ZIP7 was associated with the Golgi apparatus and was capable of transporting zinc from the Golgi apparatus into the cytoplasm of the cell.
• ZIP7 rat: 468aa; mouse: 476aa; human: 469aa - 51kD; Chromosome: 6p21.3.
• It is highly expressed in Placenta, lung, kidney and pancreas.
• Applications:Suitable for use in Western Blot, ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-10ug/ml (Chemiluminescence)ELISA: 1:100,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.