84-1176-33　Zinc Transporter ZIP4 (Solute Carrier Family 39 Member 4, Zrt- and Irt-like Protein 4, ZIP-4, hZIP4, SLC39A4, ZIP4) 100ul　Z0510-05

特徴

• Zinc is an essential nutrient for all organisms because of the many important roles this metal plays.
• Movement of zinc into and out of cells and subcellular organelles is mediated by zinc transporter proteins.
• In many organisms, zinc uptake is mediated by members of the ZIP family of metal ion transporters.
• In mammals, the Zip1, Zip2, ZIP4, Zip4, Zip5, LIV-1 (Zip6), KE4 (Zip7), and BIGM103 (Zip8) proteins have been implicated in zinc uptake in a variety of cell and tissue types.
• Zip4 (SLC39A4) belongs to the LZT subfamily.
• There are two hZIP4 isoforms, which only differ in their amino and carboxy termini.
• The largest isoform encodes a 647aa protein with an N-terminal signal peptide, whereas the shorter isoform has only 626aa.
• ZIP4 rat: 656aa; human: 647aa; mouse: 660aa - 71kD; Chromosome: 15D3.
• It is highly expressed in the small intestine and embryonic visceral yolk sac; weakly expressed in the stomach and liver.
• Found to the apical surface of enterocytes and visceral endoderm cells during zinc deficiency.
• Applications:Suitable for use in Western Blot, ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-10ug/ml (Chemiluminescence)ELISA: 1:100,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.