84-1176-31　Zinc Transporter ZIP2 (Solute Carrier Family 39 Member 2, Zrt- and Irt-like Protein 2, ZIP-2, hZIP2, SLC39A2, 6A1, Eti-1, ZIP2) 100ul　Z0510-03

特徴

• Zinc is an essential nutrient for all organisms because of the many important roles this metal plays.
• Movement of zinc into and out of cells and subcellular organelles is mediated by zinc transporter proteins.
• In many organisms, zinc uptake is mediated by members of the ZIP family of metal ion transporters.
• In mammals, the Zip1, Zip2, Zip3, Zip4, Zip5, LIV- 1 (Zip6), KE4 (Zip7), and BIGM103 (Zip8) proteins have been implicated in zinc uptake in a variety of cell and tissue types.
• ZIP2 ((SLC39A2; Eti-1; 6A1)) encodes a protein of 309aa, includes an amino terminal signal peptide of 26aa, and has the predicted topology of Zip proteins.
• The human or mouse Zip2 overexpression in transfected K562 and human embryonic kidney (HEK293) cells, respectively, increased 65Zn uptake in these cells.
• hZIP2-induced Zn uptake was shown to be highly inhibited by Mn2+ and Co2+, metals that did not substantially affect hZIP1-, mZIP1-, or mZIP2-induced Zn uptake.
• Zn uptake mediated by hZIP2 appears to be stimulated by HCO−3, non-ATP dependent, but sensitive to N-ethylmaleimide, a sulfhydryl-reactive agent.
• Applications:Suitable for use in Western Blot, ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-10ug/ml (Chemiluminescence)ELISA: 1:100,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.