Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells.
This process is termed vasculogenesis.
A further remodeling of the primitive vascular system forms the mature cardiovascular system.
This process is known as angiogenesis (sprouting of new capillary vessels from pre-existing vasculature).
Study of tumor angiogenesis has led to the identification of several proteins including basic fibroblast growth factor (bFGF) and vascular endothelial growth factor.
VEGF acts by interacting with a family of largely endothelial-specific receptor tyrosine kinases that includes VEGFR1 (flt-1/flk-2), VEGFR2 (flk- 1/KDR), and VEGFR3/Flt-4.
Disruption of VEGFRs interferes with differentiation of endothelial cells and it is lethal for the embryo.
VEGFR3 (mouse 1363aa and human 1298aa), a receptor protein tyrosine kinase, is a receptor for VEGF-C.
Mouse VEGFR3 protein topology: 1-24aa signal peptide, 25-1363 mature protein (25-775 EC domain, 776-797aa TM domain, 798-1363aa CP domain).
VEGFR3 is type 1 membrane protein.
It is expressed in adult lung, and liver, and in fetal liver, brain, intestine, and placenta.
Applications:Suitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:10,000-1:100,000; 50-100ng of control peptide/wellImmunohistochemistry: 2-20ug/ml in formaldehyde fixed tissuesOptimal dilutions to be determined by the researcher.
Control Peptide:F4601-03: Flt4, Mouse, Control Peptide (Fms-like Tyrosine Kinase 4)
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity：Recognizes mouse VEGFR3.
Isotype：IgG1
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to 20aa near the C-terminal, cytoplasmic domain of mouse VEGFR3, conjugated to KLH. Species Sequence Homology: rat; 85%, human; 73%
