84-1176-24　TWIST2 (Twist-related Protein 2, Class A Basic Helix-loop-helix Protein 39, bHLHa39, Dermis-expressed Protein 1, Dermo-1, BHLHA39, DERMO1) 100ul　T9300-08A

特徴

• Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation.
• The protein encoded by Twist gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Twist.
• It is thought that during osteoblast development this protein may inhibit osteoblast maturation and maintain cells in a preosteoblast phenotype.
• Twist and Dermo1, also called Twist1 and Twist2 respectively, were induced by a cytokine signaling pathway that required the dorsal-related protein Rela, a member of the nuclear factor kappa-B family of transcription factors, in mice.
• Twist1 and Twist2 repressed cytokine gene expression through interaction with Rela.
• TWIST proteins regulate cytokine signaling by establishing a negative feedback loop that represses the NFKB-dependent cytokine pathway.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100,000 (50-100ng control peptide/well)Western Blot: 1-10ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.