Elemental iron is required for a variety of normal cellular functions and vital for proper growth and development.
However, natural iron is quite insoluble and excess iron is harmful, since it can catalyze the formation of potentially damaging reactive oxygen species.
The major pool of body iron (~85%; 40-50mg/kg) is found in circulating hemoglobin and muscle myoglobin.
Iron absorption occurs primarily in the intestine (duodenum) and inversely related to body iron reserve.
Several proteins including Ferritin, transferrin (Tf), transferrin receptors (TfRs), and iron regulatory proteins (IRPs) etc play a key role in iron metabolism.
Transferrin (Tf, human chromosome 3, 679aa), a serum glycoprotein of ~80kD and synthesized in the liver, is the primary protein of inter-organ transport of nonheme iron.
Tf can bind two iron atoms.
Tf binds to membrane Transferrin receptors (TfRs) and taken up by endocytosis.
Iron is released from Tf, within acidic endosomes, into the cytoplasm apparently through the action of DMT1.
The apoTf-TfR complex is returned to the cell surface, where, apo-Tf dissociates from TfR at the extracellular pH.
The classical TfR, now termed TfR1, is a homodimeric (95kD subunits) type II membrane glycoprotein that binds two molecules of Tf.
Human TfR1 (human 760aa; mouse 763aa) has a cytoplasmic domain 1-67aa, 68-88aa TM, and 89-760aa as extracellular domains.
A monomeric serum form or soluble TfR1 (80kD) also exists that lacks residues 1-100aa.
Recently, a second Tf receptor, TfR2, has been cloned and characterized.
TfR2 shares 45% identity with TfR1, and 27% with PMSA.
Several variants of Tf have been identified with varying iron binding ability.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 1:5,000-1:30,000Western Blot: 1:2,000-1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：goat
Source Antibody：mouse
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide. Labeled with Biotin. F/P ratio: ~10-20:1.
