84-1176-10　Taste Receptor Type 1 Member 3 (Sweet Taste Receptor T1R3, Tas1r3, T1r3, Tr3, TS1R3) 100ul　T1024-05

特徴

• Higher vertebrates are believed to possess at least five basic tastes: Sweet, bitter, sour, salty, and umami (the taste of monosodium glutamate).
• Recently, three novel taste receptors, TR1, TR2 and TR3 have been cloned with distinct topographical distribution in taste receptor cells and taste buds.
• TR1 (rat 840aa), TR2 (rat 843aa) and TR3 (rat 858aa) share ~20% homology with each other, and ~30% with CaSR, and 22-30% with V2R pheromone receptors and mGLURs.
• TR1/TR3 responds to the umami taste stimulus (the taste of monosodium glutamate) and also to most of the 20 standard Laas, but not to their D- enantiomers or other compounds.
• TR2/TR3 recognizes diverse natural and synthetic sweeteners.
• TR3 is essential for the recognition and response to the disaccharide trehalose.
• Sequence differences within and between species can significantly influence the selectivity and specificity of taste responses.
• TR3: human: 852aa; mouse and rat: 858aa each 94.8kD; Rat chromosome: 5q36.
• Rat TR3 has a sequence identity of 92 and 72% with mouse and human TR3 proteins respectively.
• TR3 is expressed selectively in taste receptor cells but its expression has been reported in brain, liver and pancreas as well.
• Applications:Suitable for use in Western Blot, ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-10ug/ml (Chemiluminescence)ELISA: 1:100,000; 50-100ng of control peptide/wellOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.