Alzheimer’s Disease (AD) is a neurodegenerative disorder characterized by progressive loss of memory and cognition in the elderly.
An early step involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 & 43aa peptides (beta amyloid1-40, 1-42, and 1-43).
Polymerization of b-amyloid (Ab) and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition.
Ab deposits have also been found to contain 2 additional proteins termed a-synuclein and b-synuclein.
The 140aa a-synuclein is identical with non-Ab component (NACP) of AD.
The 134aa b-synuclein is homologous to 14kD bovine phosphoneuroprotein 14.
Mutations in a-synuclein gene causing a replacement of alanine with a threonine has the potential to cause the protein to misfold.
Applications:Suitable for use in Western Blot, ELISA, and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1,000-1:5,000ELISA: 1:10,000-1:100,000; 50-100ng of control peptide/wellImmunoprecipitation: 5-10ul Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes human synuclein-alpha.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 15aa located near the C-terminus of human synuclein-alpha (KLH).
