Stearoyl-CoA desaturase (SCD, also known as Delta-9 desaturase, EC 1.14.99.5) is an iron containing membrane enzyme.
It catalyzes the rate limiting step in the biosynthesis of mono-unsaturated fatty acids.
The enzyme inserts a cis double bond between the C9 and the C10 positions in the acyl-CoA derivatives of saturated fatty acids such as palmitic acid and stearic acids to produce palmitoleoyl- and oleoyl-CoA, respectively.
Mono-unsaturated fatty acids play an important role in many processes, including energy metabolism (triacylglycerol storage in adipose tissues), anti- oxidation, lipid activated signal transduction, apoptosis, mitogenesis in certain tumors, and activate certain hormone receptors.
SCD genes are tightly regulated by signals such as insulin, leptin, carbohydrate, fatty acids and temperature.
A number of SCD genes have been identified (two SCD genes in rat, and SCD1 and SCD5 in human, and SCD1-4 in mouse).
All mouse SCD genes are located on chromosome 19.
The SCD-gene products are integral membrane proteins with four TM domains containing a novel sterol regulatory element binding protein (SREBP) binding site.
The SCD enzymes have a 30aa N-terminal sequence responsible for their rapid degradation and short half-life of 3-4 hours.
Most prominent expression of SCD1 is in lipogenic tissues (liver and adipose), SCD2 in brain, SCD3 in skin, preputial and harderian gland, and SCD4 in the heart.
SCD-4: The enzyme SCD4 (mouse 352aa, ~37-40kD; 79% identity with SCD1-3) expressed is restricted in the heat.
Liver X receptor alpha (LXR-alpha) agonists and high carbohydrate fat-free diet induces SCD4 expression.
Unlike SCD1, SCd4 expression is no repressed by dietary polyunsaturated fatty acids.
Leptin deficient ob/ob mice show elevated levels of SCD4 in the heart, and leptin treatment reduced SCD4 levels without affecting SCD1 and SCD2.
SCD1 deficient mice showed elevated levels of SCD4.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1ug/mlWestern Blot: 1-10ug/ml (ECL)Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes mouse SCD4.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to 18aa of mouse SCD4, N-terminal.
