84-1176-03　SREBP-1 (Sterol Regulatory Element Binding Protein-1, SREBP1, ADD1, D630008H06, Sterol Regulatory Element Binding Transcription Factor 1, SREBF1) 100ul　S6505-15G

特徴

• Steroids are a large group of complex tetracyclic lipids that consist of a 17-carbon-ring system.
• Examples are bile acids, sterols, various hormones and saponins.
• These hormones are powerful signal molecules that regulate a host of organismal functions.
• Sterol regulatory element binding proteins (SREBPs) are membrane-bound transcription factors that control the metabolism of cholesterol and fatty acids in animal cells.
• Two SREBPs, designated SREBP-1 and SREBP-2, have been isolated and cloned from several mammalian species.
• Human SREBP-1 and -2 are ~ 50% identical in aa sequence.
• They share the tripartite structure, and they both have the capacity to activate the same genes.
• Although the two proteins can form heterodimers, this does not appear necessary for their function.
• SREBP1: Rat-1133aa; human-1147aa; human-1134aa; ~121.6kD; Chromosome 11B2.
• Isoform SREBP-1C predominates in liver, adrenal gland, brain and adipose tissue, whereas isoform SREBP-1A predominates in spleen.
• Isoform SREBP-1A and isoform SREBP-1C are found in kidney, thymus, testis, muscle, jejunum, and ileum.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100,000 (50-100ng control peptide/well)Western Blot: 1-10ug/ml (chemiluminescence)Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.