Glycolysis occurs in the cytosol, yielding 2 ATP, 2 pyruvate, and 2 NADH + 2 H+ from each glucose molecule.
The first two steps of glycolysis convert glucose-6-phosphate into a form that is easily cleaved into phosphorylated three-carbon units.
PKLR: rat, mouse, human: 574aa each - 62.3kD; Mouse chromosome: 3F1.
Highly expressed in liver and RBCs.
The PKLR gene codes for both the liver and red blood cell isozymes.
The PKM2 gene, located on chromosome 15q22, encodes 2 muscle-specific isoforms, M1 and M2.
The PK enzyme functions as a homotetramer.
Studies with the rat enzyme suggested that the red cell type is longer than the liver type in the 5-prime terminal end, but the rest of the sequence is very similar.
deduced protein sequence was similar to that of L-type PK, with an additional 31aa at the N terminus.
Addiitonal studies demonstrated that the difference between the L- and R-type pyruvate kinases is due to tissue-specific mRNAs, likely resulting from differential processing of a common nuclear RNA precursor.
Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA).
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml (chemiluminescence)ELISA: 1:100,000 (50-100ng control peptide/well.)Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes human PKLR. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 17aa peptide of human PKLR (accession #P30613), N-terminal (KLH).
